May 11 – 13, 2022
South Dakota Mines
US/Mountain timezone
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Analysis of bacterial carbonic anhydrase for accelerated carbon sequestration

May 11, 2022, 3:58 PM
1m
EEP 251 A+B (SD Mines)

EEP 251 A+B

SD Mines

Poster Biology Poster Session

Speaker

Ms Jasmeet Kaur

Description

The growing population and industrialization have led to the emission of greenhouse gases, amongst which carbon dioxide is the most persistent in the environment. This gas causes an elevation in the earth’s temperature with serious effects on health as well. The most convenient solution to this problem is carbon capture, utilization, and storage (CCUS). Carbon dioxide sequestration in the form of a stable, environmentally safe solid carbonate has obvious potential for long-term carbon dioxide storage. Microbial carbonic anhydrase (CA) catalyzes reversible carbon dioxide hydration and produces Ca/Mg carbonates that resemble weathering/carbonation in nature and are gaining merit for CCUS. The produced carbonates are environmentally stable. As a result, the variety and specificity of CAs from various microbes may be investigated for CCUS. To enable this, the present study enriched several different rock samples for bacterial growth to explore CAs for mineral carbonation. DNA and RNA are isolated to proceed with
metagenomics, meta-transcriptomics, and targeted CA sequencing. Further, universal primers are currently being designed for CAs to amplify from the isolated DNA.

Primary author

Co-authors

Bret Lingwall Rajesh Sani (Department of Chemical and Biological Engineering & CNAM-Bio Center, South Dakota School of Mines and Technology, Rapid City, SD, USA) Ms Tanvi Govil Ms Magan Vaughn

Presentation materials